LDHA, His-tag Recombinant

Catalog #

71081

$370 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human lactate dehydrogenase A (LDHA), GenBank Accession No. NM_005566), full length [2-332(end)] with N-terminal His-tag, MW= 37.5kDa, expressed in an E. coli expression system.

This product has been cited 2 times.

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Synonyms

Lactate dehydrogenase A, LDHA, LDH

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations2

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 160 mM imidazole, 0.04% Tween-20, and 20% glycerol.

37.5 kDa

Amino Acids

2–332(end)

Specific Activity

≥700 nmole/min/µg.

Genbank #

NM_005566

UniProt #

P00338

Tag(s)

N-terminal His-tag

References

1. Rong Y., et al. Tumour Biol. 2013. 34(3):1523-30.
2. Tang H., et al. Biol Reprod. 2013. 88(4):96.

Storage/Stability

>6 months at –80°C

Assay Conditions

Reaction was conducted in 100 mM Tris, pH 7.4, 2 mM sodium pyruvate, 0.2 mM β-NADH, 0.02% BSA, and LDHA in a volume of 200 µL. Initiate reactions with LDHA. Monitor the decrease in NADH absorbance at 340 nm at 37°C for 5 min. Read at Abs340 using Tecan Infinite 1000.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Metabolic Enzymes-Dehydrogenase

Regulatory Status

Research Use Only

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Datasheets/Protocols

71081.pdf

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