LAIR1 CHO Cell Line

Catalog #

78913

$4,400 *

Size: 2 vials

Qty

Bulk/Custom

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Description

LAIR1 CHO Cell Line is a clonal CHO cell line stably expressing full-length human LAIR1 (leukocyte-associated immunoglobulin-like receptor 1) (NM_002287.6). Surface expression of LAIR1 was confirmed by flow cytometry. This stable clonal cell line was selected for high levels of LAIR1 expression compared to the parental CHO-K1 cell line.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Cell surface expression of LAIR1 in LAIR1 CHO Cell Line.

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 3	BPS Bioscience #60186
Growth Medium 3J	BPS Bioscience #79974

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

LAIR1, also known as leukocyte-associated immunoglobulin-like receptor 1 or CD305 (cluster of differentiation 305) is an inhibitory receptor present in NK (natural killer) cells, T and B cells. In NK cells it binds to SHP-1 (Src homology 2 domain-containing protein tyrosine phosphatase 1) and SHP-2 phosphatases, resulting in inhibition of NK-mediated cytotoxicity. LAIR1 contributes to CD8⁺ T- cell exhaustion in cancer, by binding to collagen and suppressing T cell activity via SHP-1. The increased expression of collagen and LAIR1 in lung cancer patients is linked to a poor prognostic. In addition, patients that develop resistance to PD-1 (programmed death 1) inhibitor treatment present LAIR1 expression. The use of inhibitors that prevent the binding of LAIR1 to its ligand has been shown to increase anti-tumor immunity. Further studies are needed to fully elucidate the role of LAIR1 in cancer and develop efficacious therapies targeting LAIR1 alone or in combination with PD-1 inhibitors.

References

Meyaard L., et al., 1997 Immunity 7(2): 283-90.

Aung T., et al., 2023 Cancer Res Commun. 3(3): 471-482.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 3 (BPS Bioscience #60186): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 3J (BPS Bioscience #79974): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 5 µg/ml of Puromycin

Applications

Screen and validate antibodies against LAIR1 for drug discovery and research.
Perform binding assays to screen for potential LAIR1 ligands.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy Targets

Regulatory Status

Research Use Only