JMJD2B (KDM4B), GST-tag Recombinant

Catalog #

50111

$465 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No. NM_015015), a.a. 2-500 with N-terminal GST-tag, MW=83 kDa, expressed in Sf9 cells using a Baculovirus expression system.

This product has been cited 3 times.

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Synonyms

JHDM3B, KDM4B

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

JMJD2B (KDM4B) inhibition curve using an AlphaLISA assay (#50414-2). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations3

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥68%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mm KCl, 3 mM DTT, 20% Glycerol.

83 kDa

Amino Acids

2–500

Specific Activity

0.32 pmole/min/µg

Genbank #

NM_015015

UniProt #

O94953

Tag(s)

N-terminal GST-tag

References

1. Fodor, B.D., et al. (2006). Genes Dev. 20(12): 1557–1562.
2. Beyer S., et al. (2008). J. Biol. Chem. 283(52):36542-52.

Storage/Stability

At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Assay Conditions

10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD2B (2 – 10ng) added to the wells. Add antibody against di-methylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Demethylase

Regulatory Status

Research Use Only