IL-15 Responsive Luciferase Reporter Cell Line

Catalog #

78402

$10,175 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

This recombinant Jurkat cell line is a biologically relevant system to measure activation of the IL-15 cytokine receptor by IL-15. The cells were engineered for constitutive expression of human CD122 (IL-15Rβ; IL-2Rβ; NM_000878.4), and conditional expression of firefly luciferase driven by STAT5 response elements located upstream of the minimal TATA promoter. Expression of CD122 allows formation of a functional IL-15 receptor at the surface of Jurkat cells, which naturally express high levels of CD132 (also known as IL-15 receptor subunit γc). Activation of the STAT5 signaling pathway in response to IL-15 or IL-15 analogs can be monitored by measuring luciferase activity.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of IL-15 signaling without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Expression levels of transfected CD122 (IL-15Rβ) and of endogenous CD132.

IL-15 agonist stimulation of luciferase activity.

Dose-response of Tofacitinib effect on the IL-15 mediated cellular signaling pathway.

Product Info

Storage and Usage

Citations

Host Cell Line

Jurkat cells (clone E6-1), Human T lymphoblast, suspension

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience, #79796)

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2A	BPS Bioscience #60190

Materials Required for Cellular Assay

Name	Ordering Information
Recombinant Human IL-15 (rhIL-15)	Peprotech #200-15
Assay Medium: Thaw Medium 2	BPS Bioscience #60184
96-well tissue culture treated, white, clear-bottom assay plate	Corning #3610
ONE-Step™ luciferase assay system	BPS Bioscience #60690
Luminometer

UniProt #

P40933

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

IL-15 is a proinflammatory cytokine mainly produced in dendric cells (DC) and monocytes. It remains associated with IL-15Rα on the surface of DC and monocytes, and binds to the CD122/CD132 heterodimeric receptor present at the surface of various target immune cells, including CD8⁺ memory T cells, resulting in their proliferation and differentiation. Although there is no indication that unassociated IL-15 exists in vivo, soluble recombinant IL-15 and its analogs have been examined for their therapeutic potential.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 2 (BPS Bioscience #60184): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2A (BPS Bioscience #60190): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 200 μg/ml of Hygromycin and 1 mg/ml Geneticin.

Applications

Characterize recombinant IL-15, IL-15 analogs or other agonists of IL-15R
Screen Janus kinase or IL15R inhibitors in a cell-based assay

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only