Human Thrombopoietin Recombinant

Catalog #

90249-A

$130 *

Size: 2 µg

Qty

Bulk/Custom

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Description

Recombinant TPO is disulfide-linked monomer protein consisting of 332 amino acid residues, and migrates due to glycosylation as an approximately 35 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human TPO erythropoeitin domain was expressed in Chinese Hamster Ovary cells.

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Synonyms

TPO, Thrombopoietin, MGDF, Megakaryocyte Growth And Development Factor, MKCSF, TSF, thrombopoiesis stimulating factor

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

CHO

Purity

≥98% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from a 0.2 µm filtered PBS solution, pH 7.

35 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

22–353

Biological Activity

The ED₅₀ was determined by the dose-dependent proliferation of Mo7e cells to be in the range of 1.0-2.0 ng/ml.

Genbank #

P40225

UniProt #

P07202

Background

Thrombopoietin (TPO), known also as TSF (thrombopoiesis stimulating factor), for mediator substances that function as natural stimulators of megakaryocytopoiesis and thus, promote the differentiation of platelets. TPO stimulates an increase in the sizes and numbers of megakaryocytes. It also increases the number of small acetyl-cholinesterase positive cells that are early precursor cells of the megakaryocytic lineage. TPO is a protein of 332 amino acids that shows significant homology with Epo (erythropoietin) in its N-terminal domain. A comparison of human and porcine TPO sequences shows 83 % identity between the erythropoietin-like domains but only 67 % between the C- terminal domains.

References

1. Lupia E, et al. Mediators Inflamm. 2012,2012:390892.
2. Basciano PA, Bussel JB. Curr Opin Hematol. 2012 Sep,19(5):392-8.
3. de Graaf CA, Metcalf D. Cell Cycle. 2011 May 15,10(10):1582-9.

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Datasheets/Protocols

90249-A Lot 221021

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Human Thrombopoietin Recombinant

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