Human Interleukin-12 Recombinant

Catalog #

90178-B

$205 *

Size: 10 µg

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Description

Recombinant Interleukin-12 is a disulfide-linked heterpdimer protein consisting of 306 amino acid residue p40 subunit and a 197 amino acid residue p35 subunit, and migrates due to glycosylation as an approximately 75 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Interleukin-12 mature chain was expressed in Chinese Hamster Ovary (CHO) cells.

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Synonyms

IL-12, IL12, Interleukin-12, NKSF, CLMF, NFSK, Cytotoxic Lymphocyte Maturation Factor

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Hamster

Purity

≥97% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from 0.2 µm filtered PBS, pH 7.5.

19 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

23–529

Biological Activity

The ED₅₀ was determined by the dose-dependent proliferation of activated human T-lymphoblasts. cells was found to be in the range of 0.1-0.2 ng/ml.

Genbank #

P29460

UniProt #

P29460

Background

IL-12 is secreted by peripheral lymphocytes after induction. It is produced mainly by B-cells and to a lesser extent by T-cells. The most powerful inducers of IL-12 are bacteria, bacterial products, and parasites. IL-12 is produced after stimulation with phorbol esters or calcium ionophore by human B-lymphoblastoid cells. The IL-12 receptor, CD212, appears to be a single protein of approximately 110 kDa. Up to 1000-9000 high affinity IL-12 receptors/cell are expressed on peripheral blood mononuclear cells activated by various mitogens for T-cells or by IL2. IL-12 receptors are present on activated T-cells expressing CD4 and CD8 and on activated CD56(+) natural killer cells. Resting peripheral blood mononuclear cells, tonsillar B-cells, or tonsillar B-cells activated by anti-IgM/Dx, anti-IgM/Dx + IL-2, or SAC + IL-2 do not express the receptor. Human IL-12 is not active in murine lymphocytes. Hybrid heterodimers consisting of murine p35 and human p40 subunits retain bioactivity on murine cells, however, the combination of human p35 and murine p40 is completely inactive on murine cells. Murine IL-12 is active on both murine and human lymphocytes.

References

1. J. Immunol., Jan 2003, 170: 597 - 603.
2. J. Immunol., May 2000, 164: 4752 - 4761.
3. J. Immunol., Jan 2000, 164: 839 - 847.

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Human Interleukin-12 Recombinant

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