HDAC8, His-Tag Recombinant

Catalog #

50008

$460 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human histone deacetylase 8 (HDAC8), full length encompassing amino acids 1-377(end). This construct contains a C-terminal His-tag. The recombinant protein was affinity purified.

This product has been cited 73 times.

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Synonyms

Histone deacetylase 8, HD8, Protein deacetylase HDAC8, Protein decrotonylase HDAC8, HDACL1, CDA07, HDAC-8

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

HDAC8 Activity

HDAC8 inhibition curves using a fluorogenic assay (#50068). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations73

Species

Human

Construct

HDAC8 (1-377(end)-His)

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, variable Imidazole, and 20% glycerol

43 kDa

Amino Acids

1-377(end)

Specific Activity

≥300 pmol/min/µg

Genbank #

NM_018486

UniProt #

Q9BY41

Tag(s)

C-terminal His-tag

References

1. Lee, H. et al. Mol. Cell. Biol. 26 (14), 5259-5269 (2006).
2. Gantt,S.L. et al., Biochemistry 45 (19), 6170-6178 (2006).

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 20 µM Fluorogenic HDAC class 2a substrate (BPS Bioscience #50040), and HDAC8 (3.1 – 200 ng). Incubation condition: 30 min at 37°C followed by developing for 15 min at room temperature.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Deacetylase

Regulatory Status

Research Use Only