HDAC5, Catalytic Domain, His-tag Recombinant

Catalog #

50005

$440 *

Size: 10 µg

Qty

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Description

Human HDAC5 catalytic domain , GenBank Accession No. NM_005474, a.a. 656-1122 (end) with C-terminal His-tag, MW= 51 kDa, expressed in baculovirus expression system.

This product has been cited 19 times.

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Synonyms

histone deacetylase 5, HDAC-5

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

HDAC5 inhibition curves using a fluorogenic assay (#50065). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations19

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 imidazole, and 10% glycerol.

51 kDa

Amino Acids

656–1122 (end)

Specific Activity

≥1000 pmol/min/µg.

Unit Definition

One U =1 pmol of acetyl group removed/min/µg of enzyme

Genbank #

NM_005474

UniProt #

Q9UQL6

Tag(s)

C-terminal His-tag

References

1. Spiegelberg, B.D. and Hamm, H.E. (2005) J. Biol. Chem. 280, 41769-41776
2. Waltregny et al., (2004) J. Neural Transm. 48, 273-290.

Storage/Stability

>6 months at -80°C.

Assay Conditions

25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 30 µM BPS HDAC class 2a substrate , BPS Catalog number 50040, and HDAC5. Incubation condition: 30 min at 30°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Deacetylase

Regulatory Status

Research Use Only

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Datasheets/Protocols

50005_Lot100414.pdf 50005_Lot140211.pdf

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HDAC5, Catalytic Domain, His-tag Recombinant

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