GPC3 – CHO-K1 Recombinant Cell Line

Catalog #

78100

$7,150 *

Size: 2 vials

Qty

Bulk/Custom

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Description

Recombinant CHO-K1 cells expressing GPC3 (Glypican 3), a cell surface proteoglycan bearing heparin-sulfate; ref. seq. NM_004484.2.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Glypican-3 cell line, OCI5 recombinant cell line

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Product Data Gallery

FACS Analysis

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1

Species

Human

Supplied As

Each vial contains ~ 2 x 10⁶ cells in 1 ml of 10% DMSO in FBS.

Materials Required But Not Supplied

Materials for Cell Culture

Thaw Medium 3 (BPS Bioscience #60186)
Growth Medium 3J (BPS Bioscience #79974)

Materials Required for Cellular Assay

Human TruStain FcX™ (Fc Receptor Blocking Solution) (Biolegend #422302)
Human Glypican 3 APC-conjugated Antibody (R&D Systems #FAB2119A)
Flow Cytometer

UniProt #

P51654

Mycoplasma Testing

The cell line has been screened using the MycoAlert™ Mycoplasma Detection kit (Lonza, #LT07-218) to confirm the absence of Mycoplasma species.

Background

GPC3, also known as Glypican-3 and OCI5, belongs to the glypican family and is highly expressed in the lungs, liver and kidneys. Its function is tissue dependent and can either promote or suppress tumorigenesis. Being a heparin sulfate proteoglycan, it is overexpressed in neoplasms including malignant melanoma, hepatocellular carcinoma, and testicular yolk sac tumors and plays a significant role in cell growth and differentiation.

Storage/Stability

Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Culture Conditions

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 3 (BPS Bioscience #60186): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 3J (BPS Bioscience #79974): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 5 μg/ml of Puromycin

Shipping Temperature

-80°C

Notes

License Disclosure

Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide

Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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Datasheets/Protocols

78100

SDS

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Certificate of Analysis (CoA)

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GPC3 – CHO-K1 Recombinant Cell Line

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