Fucokinase, FLAG-tag Recombinant

Catalog #

71077

$515 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human fucokinase (GenBank Accession No. NM_145059), a.a. 2-1084(end) with N-terminal FLAG-tag, MW = 118.6 kDa, expressed in baculovirus using an Sf9 insect cell expression system.

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Synonyms

fucokinase, FuK

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 20% glycerol, and 80 ng/µl FLAG peptide

118.6 kDa

Amino Acids

2–1084(end)

Specific Activity

≥85.3 pmole/min/µg

Genbank #

NM_145059

UniProt #

Q8N0W3

Tag(s)

N-terminal FLAG-tag

References

1. Hinderlich, S., et al., Biochem Biophys Res Commun. 2002 Jun 14; 294(3):650-4.
2. Zeitler, R., et al. J. Enz. Inhib. 1997 Feb; 11(4):265-73

Storage/Stability

>6 months at –80°C

Assay Conditions

The reaction mixture contained 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 2 mM L-Fucose, 100 μM ATP, and various amount of fucokinase in a final volume of 50 μL. The reaction was incubated at 37°C for 1 hr, and the amount of ATP consumed was calculated using Kinase-Glo Plus^® reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Metabolic Enzymes, Carbohydrate Kinase

Regulatory Status

Research Use Only

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Datasheets/Protocols

71077_Lot130129.pdf

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Fucokinase, FLAG-tag Recombinant

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