Fluorogenic SHP1 (PTPN6) Assay Kit

Catalog #

79831

$440 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Fluorogenic SHP1 Assay Kit is designed to measure SHP1 activity for screening and profiling applications, in a homogeneous assay with no timeconsuming washing steps. The SHP1 assay kit comes in a convenient 96-well format, with purified SHP1 enzyme, fluorogenic substrate, and PTP assay buffer for 100 enzyme reactions. This kit is developed to identify inhibitors of the regulatory region of SHP1.

Need us to run inhibitor screens or profile your compounds against SHP1 (PTPN6)? Check out our Protein Tyrosine Phosphatase Screening Services.

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Synonyms

SHP1; SHP-1; HCP; HCPH; HPTP1C; PTP-1C; SHP-1L; SH-PTP1, SHP

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Fluorogenic

Materials Required But Not Supplied

Fluorescent microplate reader capable of reading exc/em=360nm/460nm

Format

Catalog #	Component	Amount	Storage
100342	Recombinant Human SHP1 (221-523)	≥1 µg	-80°C	Avoid freeze/ thaw cycles!
	0.5 mM PTP Substrate (DiFMUP)	50 µl	-80°C
79716	5X PTP Assay Buffer	3 ml	-20°C
	0.5 M DTT	200 µl	-20°C
79685	Black, low binding black microtiter plate	1	Room Temp

UniProt #

P29350

Background

SHP1 (also known as PTPN6) is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine-stimulated cells. The noncatalytic N-terminus of this phosphatase can interact with MAP kinases and negatively regulates ERK2 and p38 MAP-kinases activity. The PTPN6 is involved in the regulation of T cell antigen receptor (TCR) signaling, which is thought to function through dephosphorylating molecules related to the MAP kinase pathway.

References

Gee, K.R., et al., Anal Biochem, 1999 Aug 15; 273(1):41-8.
Kilgore, N.E., et al., J. Immunol. 2003, 170:4891-4895.
Duchesne, C., et al. J. Biol. Chem 2003, 278:14274-14283.

Storage/Stability

6 months from date of receipt when stored as directed.

Applications

Great for studying enzyme kinetics and HTS applications.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Phosphatase

Regulatory Status

Research Use Only