FDH, (P. putida), His-tag Recombinant

Catalog #

71076

$370 *

Size: 100 μg

Qty

Bulk/Custom

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Description

Pseudomonas putida formaldehyde dehydrogenase (FDH) a.a. 2 – 399 (end) (GenBank Accession No. D21201) with N-terminal His-tag, MW = 42.9 kDa, expressed in an E. coli expression system.

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Synonyms

formaldehyde dehydrogenase, FDH

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Pseudomonas putida

Host Species/Expression System

E. coli

Purity

≥74%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol

42.9 kDa

Amino Acids

2 – 399 (end)

Specific Activity

≥35 µmol/min/µg

Genbank #

D21201

UniProt #

P46154

Tag(s)

N-terminal His-tag

References

1. Ito, K., et al., J Bacteriol. 1994;176:2483-2491.
2. Tanaka, K., et al., J Mol Biol. 2002;324:519-533.

Storage/Stability

>6 months at –80°C

Assay Conditions

The reaction was monitored by measuring NADH production by following absorbance at 340nm at room temperature for 5min. Buffer: pH 7.3, 20 mM Tris, 50 mM NaCl, 20 µM Fe+2, 500 mM a-ketoglutarate, 2 mM ascorbic acid, 0.01% Tween-20, and 1 mM NAD+.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Metabolic Enzymes-Dehydrogenase

Regulatory Status

Research Use Only

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Datasheets/Protocols

71076_Lot121211.pdf

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FDH, (P. putida), His-tag Recombinant

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