Diablo, His-Tag Recombinant

Catalog #

101118

$320 *

Size: 100 µg

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Description

Recombinant human Diablo (direct IAP binding protein with low pI), transcript 1, encompassing amino acids 56-239(end). This construct contains a C-terminal His-tag (6xHis). This protein was affinity purified.

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Synonyms

Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase,Diablo IAP-binding mitochondrial protein, SMAC

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

XIAP:SMAC Peptide Binding Assay

Product Info

Storage and Usage

Citations

Species

Human

Construct

Diablo (56-239(end)-His)

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and variable Imidazole

22 kDa

Amino Acids

56-239(end)

Genbank #

NM_019887.6

UniProt #

Q9NR28

Tag(s)

C-terminal His-tag

Background

Diablo (direct IAP binding protein with low pI), also named SMAC (second mitochondria-derived activator of caspases), is a ubiquitously expressed mitochondrial protein involved in apoptosis. Under normal conditions this protein is found in the intermembrane space of the mitochondria. However, when a cell undergoes apoptosis, the mitochondrial membrane becomes porous and Diablo moves the cytosol. Once in the cytosol it can bind and inhibit IAPs (inhibitor of apoptosis protein) proteins, allowing caspase activation. This protein has been linked to cancer, and overexpression of Diablo may sensitize cancer cells to apoptosis. The use of Diablo mimetics, alone or in combination with other therapeutical approaches, is a promising cancer therapy.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

A neutravidin coated plate was coated with 7 ng/well of SMAC/Diablo Peptide [AVPIAQKSE], Biotin-Labeled (InnoPep #1261-010) overnight. After washing and blocking, 25 ng/well of 8.75 nM XIAP, FLAG-Tag Recombinant (BPS Bioscience #80401) and varying concentrations of Diablo were added to the wells and incubated for 1 hour. Binding between XIAP and SMAC Peptide in the presence of increasing concentrations of Diablo was detected by incubation with 1:1000 dilution of DYKDDDDK Epitope Tag Antibody (FG4R) (Novus Biologicals #NBP2-37823) for 45 minutes, followed by 1:5000 dilution of HRP-Secondary Antibody 1 (Mouse) for 30 minutes.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for binding assays with XIAP.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Apoptosis

Regulatory Status

Research Use Only

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Datasheets/Protocols

101118 Lot 230922-4

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