CTLA4 - HEK293 Recombinant Cell Line

Catalog #: 60681
Size: 2 vials
CTLA4 is a member of the immunoglobulin superfamily. It is expressed by activated T cells and transmits an inhibitory signal to T cells. CTLA4 is homologous to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells. CTLA-4 binds CD80 and CD86 with greater affinity and avidity than CD28 thus enabling it to outcompete CD28 for its ligands and act as an “off” switch when bound to CD80 or CD86. Not surprisingly, CTLA-4 is an important drug target for the regulation of the host’s response to cancer.
Recombinant HEK293 stably expressing human CTLA4 (Cytotoxic T-Lymphocyte Associated Protein; CD152), GenBank Accession #NM_005214.
Cytotoxic T-lymphocyte-associated protein 4, CD152
Supplied As:
Each vial contains ~3 X 106 cells in 1 ml of 10% DMSO.
Host Cell line:
HEK293 cells
Mycoplasma Testing:
The cell line has been screened using the metabolite-based Mycoplasma Detection Kit (Biotool, #B3903) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 1 (BPS Bioscience, #60187): MEM medium (Hyclone, #SH30024.01) + 10% FBS (Life Technologies, #26140-079) + 1% non-essential amino acids (Hyclone, #SH30238.01) + 1 mM Na pyruvate (Hyclone, #SH30239.01) + 1% Penicillin/Streptomycin (Hyclone, SV30010.01)

Growth Medium 1F (BPS Bioscience #79540): Thaw Medium 1 (BPS Bioscience, #60187plus 100 µg/ml of Hygromycin B (Life Technologies, #10687-010). 

Cells should be grown at 37°C with 5% CO2 using Growth Medium 1F to ensure recombinant expression. CTLA4-HEK293 cells should display a typical cell division time of about 24 hours. 

Instructions for use:
See cell line data sheet for detailed culturing and assay protocol.
Storage / Stability:

Immediately upon receipt, store in liquid nitrogen.

• Screening for CTLA4 binding molecules (such as anti-CTLA4 antibody) in a cellular context

License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Avoid freeze/thaw cycles
Scientific Category:
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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