Chemi-Verse™ PAK7 Kinase Assay Kit

Catalog #

82183

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Chemi-Verse™ PAK7 Kinase Assay Kit is designed to measure PAK7 (p21-activated kinase 7) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant PAK7 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

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Synonyms

PAK5; KIAA1264; MGC26232

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Product Data Gallery

Inhibition of PAK7 kinase activity by Staurosporine.

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
	PAK7*	1 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
	PAKtide, 1 mg/ml	500 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

Q9P286

Background

PAK7 (p-12-activated kinase 7), also known as PAK5, is a member of the PAK family of serine/threonine kinases. The PAK family is highly evolutionary conserved and regulates cell survival, apoptosis, cytoskeleton dynamics, and other critical cellular functions. PAK7 belongs to group II, which can be activated by Cdc42 (cell division control protein 42) only, as opposed to group I, which depends on either Rac1 or Cdc42.I It is found mostly in the brain, where it participates in neurite outgrowth and microtubule stabilization. Deletion of PAK7 in mice results in defects in memory, learning and movement. PAK7 is regulated by Aurora kinase A, both being upregulated in esophageal squamous carcinoma. In turn, PAK7 phosphorylates proteins like synaptojanin-1, pacsin-1, GATA1 and p120-catenin. PAK7 has also been identified as a risk factor for psychosis, which may result from abnormal synapse development and plasticity. Upregulation of PAK7 has been found in several cancer types, such as gastric, breast, ovarian cancer, osteosarcoma and others, where it also contributes to cell survival and resistance to chemotherapy. The development of specific inhibitors targeting PAK7, to be used alone or in combination therapy, will greatly benefit the cancer therapy field.

References

Wu A. and Jiang J., 2022 Leukemia 36:315-326.
Morris D., et al., 2014 Hum Mol Genet 23(12):3316-3326.
Gu Y.-F. and Kong L.T., 2021 Human & Experimental Toxicology 40(12): 2202-2214.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinases

Regulatory Status

Research Use Only

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Chemi-Verse™ PAK7 Kinase Assay Kit

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