CD45(PTPRC), His-tag Recombinant

Catalog #

30044

$325 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Protein tyrosine phosphatase CD45, also known as PTPRC (GenBank accession No. NM_080922), catalytic domain, a.a. 632-1304 (end) with N-terminal His-tag, MW=81 kDa, expressed in a Baculovirus Sf9 expression system.

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Synonyms

CD45, LCA, T200, LY5, B220, GP180

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol and 3 mM DTT.

81 kDa

Amino Acids

632–1304

Specific Activity

341 pmole/min/μg

Genbank #

NM_002838

UniProt #

P08575

Tag(s)

N-terminal His-tag

Background

PTPRC (CD45) contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. PTPRC has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling.

References

1. Rachmilewitz, J. et al., J. Biol. Chem. 278, 14059-14065.
2. Fernandis, A.Z. et al., J. Biol. Chem. 278, 9536-9543.

Storage/Stability

>12 months at -80°C.

Assay Conditions

20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM PNPP, 20 min at room temperature. Absorbance was measured at 405 nm. Specific activity was calculated based on pnp=18,000M-1cm-1.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

PTP/Receptor

Regulatory Status

Research Use Only

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Datasheets/Protocols

30044_Lot150220-D.pdf 30044_Lot130829-G1.pdf

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CD45(PTPRC), His-tag Recombinant

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