CD34 CHO Recombinant Cell Line

Catalog #

78336

$7,150 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant clonal CHO stable cell line constitutively expressing full length human CD34 protein. The surface expression of CD34 in this cell line was validated by flow cytometry.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Hematopoietic progenitor cell antigen CD34, CD_antigen CD34

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Product Data Gallery

Confirmation of CD34 expression on CHO-K1 recombinant cells

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent

Species

Human

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of 10% DMSO in FBS

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 3	BPS Bioscience #60186
Growth Medium 3D	BPS Bioscience #79539

UniProt #

P28906

Mycoplasma Testing

The cell line has been screened using the MycoAlert™ Mycoplasma Detection kit (Lonza, #LT07-218) to confirm the absence of Mycoplasma species.

Background

CD34 (Cluster of Differentiation-34) is a single-pass transmembrane phosphoglycoprotein and a stem cell antigen. It is a receptor for L-Selectin (CD62L) and CrkL ligands, as well as E- and P-Selectin in the bone marrow extracellular matrix. It can act both as a cell adhesion molecule and as an adhesion blocking molecule against mast cells, dendritic precursors and eosinophils. CD34 is most commonly characterized by its expression on the surface of hematopoietic stem cells (HSC), and is therefore utilized for positive selection and counting of CD34⁺ cells in clinical bone marrow transplants. CD34^(-) HSCs have also been identified, which exhibit a distinct progenitor profile. CD34 bears a controversial identity as a mesenchymal stromal cell (MSC) marker, the evidence for which is highly context-dependent. In addition, CD34 is expressed by other progenitor cell types such as quiescent keratocytes, stem cells within the hair follicle that replace epithelial layers of the skin, and within sub-populations of interstitial cells of Cajal (ICC). More recently CD34 has been identified as a potential predictor of neoadjuvant chemotherapy efficacy in cervical cancer patients, and when found in tumor stroma, is associated with better colorectal cancer survival.

Storage/Stability

Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Culture Conditions

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 3 (BPS Bioscience #60186): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 3D (BPS Bioscience #79539): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 mg/ml Geneticin

Instructions for Use

See assay protocol for detailed instructions

Applications

1. Useful for screening or performing binding assays of antibodies recognizing human CD34 in a cellular context.
2. Assessment of reagents designed to characterize the lineage/identity of hematopoietic stem and other progenitor cells.
3. Development of stem cell characterization standards and procedures that include CD34^+/-

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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CD34 CHO Recombinant Cell Line

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