Bas (PTPN13), GST-tag Recombinant
Catalog #
30024
$325
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Description
Human, recombinant PTP-Bas, catalytic domain (Gene bank accession number NM_080685), a.a. 2091-2490 (catalytic domain), with N-terminal GST tag, MW=71.8 kDa, expressed in an E. coli expression system.
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Synonyms
Bas, FAP-1, PTPN13, APO-1/CD95-Associated Phosphatase 1, PTP-BAS, PNP1, PTP1E, PTPL1, PTPLE, PTP-BL, Fas-Associated Phosphatase 1
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
E. coli
Format
Aqueous buffer solution
Formulation
25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
MW
71.8 kDa
Amino Acids
2091–2490
Specific Activity
5 U/µg
Unit Definition
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Genbank #
NM_080685
UniProt #
Q12923
Background
PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN13 is a large protein that possesses a PTP domain at C-terminus, and multiple noncatalytic domains, which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains, and a leucine zipper motif. PTPN13 binds to a negative regulatory domain in Fas that inhibits Fas-induced apoptosis.
References
1. W. Kimber, et al., Biochem.J. 2003, 376: 525-535.
2. N. Kachel, et al. J.Mol. Biol. 2003, 334: 143-155.
2. N. Kachel, et al. J.Mol. Biol. 2003, 334: 143-155.