B7-H4 CHO Cell Line (Low, Medium or High Expression)

Only %1 left

Catalog #

78586

As low as $7,150 *

Cat # Size Price*

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

B7-H4 CHO Cell line is a recombinant clonal stable CHO-K1 cell line constitutively expressing full length human B7- H4 protein, also known as V-set domain-containing T-cell activation inhibitor 1 (VTCN1); (NM_024626.4). Surface expression of B7-H4 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of B7-H4 expression (Low, Medium and High), to mimic different B7-H4 expression levels in various cancer types.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

●

Synonyms

V-set domain-containing T-cell activation inhibitor 1, B7 homolog 4 (B7-H4), B7h.5, Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, VTCN1, UNQ659/PRO1291, B7H4

●

Product Data Gallery

Flow Cytometry analysis of cell surface expression of Human B7-H4 in CHO-K1 Cell lines (low, medium, and high expression).

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 3	BPS Bioscience #60186
Growth Medium 3B	BPS Bioscience #79529

UniProt #

Q7Z7D3

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

B7-H4 is an immune checkpoint that belongs to the B7 family and is encoded by the VTCN1 gene. B7-H4 is expressed on the surface of antigen presenting cells, interacting with currently unidentified ligands to negatively regulate immune cell responses and suppress tumor-associated immunity. B7-H4 also contributes to changes in the tumor microenvironment that benefit cancer cells and support their proliferation, survival, invasion, and metastasis. B7-H4 is highly expressed in some cancer cells, and has been correlated with tumor progression, particularly in ovarian and breast cancer, making it an attractive target for immunotherapy.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 3 (BPS Bioscience #60186): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 3B (BPS Bioscience #79529): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin, and 500 µg/ml of Hygromycin B.

Applications

Screen and validate antibodies against B7-H4 for drug discovery and research.
Screen for compounds or ligands that regulate or inhibit B7-H4 signaling in a cellular context.
Optimize and perform biological assays.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only