Anti–WDR5 polyclonal antibody

Catalog #

25321

$335 *

Size: 100 µl

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Polyclonal antibody raised in rabbit against mouse Wdr5 (WD (tryptophan–aspartate) repeat domain protein 5), using two KLH–conjugated synthetic peptides containing an amino acid sequence from the central part of the protein

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Synonyms

BIG3, SWD3, WDR-5

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Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Whole serum

Format

aqueous solution

Formulation

Whole antiserum from rabbit containing 0.05% azide

Concentration

100 µl

Immunogen

synthetic peptide

Background

Wdr5 (UniProtKB/Swiss–Prot entry P61964) belongs to the family of WD repeat proteins which are involved in different cellular processes such as cell cycle progression, signal transduction, apoptosis, and gene regulation. It is a component of the Set1A and Set1B histone H3 methylation complexes. These complexes methylate lysine 4 of H3, thereby activating gene transcription. Wdr5 interacts with H3 dimethyl K4, but not with tri or mono methylated H3K4.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse Wdr5 (Cat. # 25321). By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:12,300.

Western blot analysis using the antibody directed against Wdr5
Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Wdr5 (Cat. # 25321), diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (36 kDa) is indicated on the right.

Applications

ELISA (1:100 – 1:500)
WB (1:1000)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Mouse

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–WDR5 polyclonal antibody

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