Anti–RbAp 46/48 monoclonal antibody

Catalog #

25311

$335 *

Size: 50 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Monoclonal antibody raised in mouse against the full length human RbAp48 protein (Retinoblastoma–binding protein p48) fused to GST. The antibody recognizes both RbAp48 and RbAp46.

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Synonyms

RBBP7/4, RBBP–7/4, RBAP46/48, NURF 55

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Product Data Gallery

Product Info

Storage and Usage

Citations

Isotype

IgG2b

Clonality

monoclonal

Host Species/Expression System

Mouse

Purification

Protein G purified

Format

Aqueous buffer solution

Formulation

PBS containing 0.05% azide and 0.05% ProClin 300.

Concentration

50 µg/25 µl

Immunogen

recombinant protein

Background

RbAp48 (UniProtKB/Swiss–Prot entry Q09028) is a component of several complexes which regulate chromatin metabolism. These include (1) the chromatin assembly factor 1 (CAF–1) complex, which is required for chromatin assembly following DNA replication and DNA repair; (2) the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; (3) the nucleosome remodelling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; (4) the PRC2/EED–EZH2 complex, which promotes repression of homeotic genes during development; and (5) the NURF (nucleosome remodeling factor) complex. Therefore it may target chromatin assembly factors as well as chromatin remodeling factors and histone deacetylases to their histone substrates in a nucleosomal DNA dependent manner.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Western blot analysis using the monoclonal antibody directed against hRbAp46/48
Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 μg) and the monoclonal antibody against hRbAp46/48 (cat. #25311) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left), the position of the protein of interest is shown on the right.

Applications

WB (1:1000)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–RbAp 46/48 monoclonal antibody

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