Anti–PHF8 polyclonal antibody

Catalog #

25308

$335 *

Size: 50 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Polyclonal antibody raised in rabbit against human PHF8 (PHD finger protein 8), using 3 different KLH–conjugated synthetic peptides.

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Synonyms

JHDM1F, MRXSSD, ZNF422

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Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Affinity purified

Format

Aqueous buffer solution

Formulation

PBS containing 0.05% azide and 0.05% ProClin 300.

Concentration

50 µg/39 µl

Immunogen

synthetic peptide

Background

PHD finger protein 8 (UniProtKB/Swiss–Prot entry Q9UPP1) is a jumonji domain containing protein. Like other members of the jumonji family, PHF8 may therefore play a role in histone demethylation. Mutations in PHF8 lead to Siderius type X–linked mental retardation (MRXSSD), a mild to borderline type of mental retardation.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human PHF8 (Cat. # 25308), crude serum and flow-through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,500.

Western blot analysis using the antibody directed against PHF8
E14TG2A mouse embryonic stem cells were transfected with a conditional allele of PHF8. Nuclear extracts (135 µg) from wild type cells (WT) and from 2 clones (F1, B8) with an active and a targeted allele (F1ko, B8ko), respectively, were analyzed by Western blot using the antibody against PHF8 (Cat. # 25308). The antibody was diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Western blot analysis was performed by Philipp Seibert, BIOTEC, Dept. of Genomics, TU Dresden, Tatzberg 47/49, 01307 Dresden.

Applications

ELISA (1:100 – 1:500)
WB (1:1000)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human, mouse

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–PHF8 polyclonal antibody

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