Anti–MeCP2 polyclonal antibody

Catalog #

25304

$415 *

Size: 50 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Polyclonal antibody raised in rabbit against MeCP2 (Methyl–CpG–binding domain protein 2), using a KLH–conjugated synthetic peptide containing a sequence from the C–terminal part of the protein

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Synonyms

AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT

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Product Data Gallery

Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Affinity purified

Format

Aqueous buffer solution

Formulation

PBS containing 0.05% azide and 0.05% ProClin 300.

Concentration

50 µg/42 µl

Immunogen

synthetic peptide

Background

MeCP2 (UniProt/Swiss–Prot entry P51608) is a chromosomal protein with abundant binding sites in the chromatin. It belongs to the family of methyl CpG binding proteins which also comprises MBD1, MBD2, MBD3 and MBD4. MeCP2 can bind specifically to methylated promoters, thereby repressing transcription. This transcriptional repression is mediated through interaction with histone deacetylase and the corepressor SIN3A. MeCP2 also is essential for development. Mutations in MeCP2 are the cause of several types of mental retardation including Rett syndrome, a progressive neurological disorder that causes mental retardation in females and mental retardation syndromic X–linked type 13, and may also be involved in Angelman syndrome and susceptibility to some types of autism.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

ChIP results obtained with the antibody directed against MeCP2
ChIP assays were performed using human osteosarcoma (U2OS) cells, the antibody against MeCP2 (Cat. # 25304) and optimized PCR primer sets. Sheared chromatin from 1x10⁶ cells and 5 µg of antibody were used per ChIP experiment. IgG (1 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ZMYND10 gene (used as a positive control) and CDC6 gene (used as a negative control). Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against MeCP2 (Cat. # 25304) and the crude serum. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the purified antibody was estimated to be: 1:32,900.

Western blot analysis using the antibody directed against MeCP2
Nuclear extracts (40 µg) from HeLa cells were analyzed by Western blot using the antibody against MeCP2 (Cat. # 25304) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Applications

ChIP (5 µg/ChIP)
ELISA (1:1000)
WB (1:1000)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human

Notes

The optimal antibody amount per IP should be determined by the end–user. We recommend testing 1–5 µg per IP

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–MeCP2 polyclonal antibody

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