Anti–Cre polyclonal antibody

Catalog #

25215

$335 *

Size: 100 µl

Qty

Bulk/Custom

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Description

Polyclonal antibody raised in rabbit against Cre recombinase using a KLH–conjugated synthetic peptide located at the C–terminal part of the protein

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Synonyms

Cre recombinase

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Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Whole serum

Format

aqueous solution

Formulation

Whole antiserum from rabbit containing 0.05% azide

Concentration

100 µl

Immunogen

synthetic peptide

Background

Cre recombinase originates from bacteriophage P1 and recognizes loxP sites in the genome. It catalyzes recombination between two loxP sites, thereby excising the intervening DNA sequence. Cre recombinase is used as a tool for the generation of transgenic animals

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Determination of the antibody titer
To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against Cre (cat. No. 25215) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,900.

Western blot analysis using the antibody directed against Cre (1)
Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the antibody against Cre (cat. No. 25215), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 38 kDa) is indicated on the right.

Applications

ELISA (1:100)
WB (1:500)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Datasheets/Protocols

25215 Lot 220819

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Anti–Cre polyclonal antibody

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