Anti–Chd1 polyclonal antibody

Catalog #

25213

$335 *

Size: 50 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Polyclonal antibody raised in rabbit against human CHD1 (Chromodomain helicase DNA binding protein 1), using a GST–fused recombinant protein.

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Synonyms

CHD–1, ATPase DNA–binding protein 1

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Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Affinity purified

Format

Aqueous buffer solution

Formulation

PBS containing 0.05% azide and 0.05% ProClin 300.

Concentration

50 µg/25 µl

Immunogen

recombinant protein

Background

CHD1 (UniProtKB/Swiss–Prot entry O14646) is an ATP–dependent chromatin remodeling factor, containing two tandem chromodomains. In human both chromodomains are essential for specific binding to histone H3, methylated at lysine 4. CHD1 belongs to the SNF2/RAD54 helicase family and contains also one helicase ATP–binding domain and one helicase C–terminal domain. It may play an important role in gene regulation.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Western blot analysis using the antibody directed against CHD1
Western blot was performed on nuclear extracts from HeLa cells (HeLa nuclear extracts, 20 μg) with the antibody against CHD1 (Cat. No. 25213) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.

Applications

WB (1:500)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–Chd1 polyclonal antibody

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