Anti–3–mC polyclonal antibody

Catalog #

25200

$360 *

Size: 50 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Polyclonal antibody raised in rabbit against 3–methylcytosine (3–mC) conjugated to BSA.

●

Product Data Gallery

Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Protein G purified

Format

Aqueous buffer solution

Formulation

PBS containing 0.05% azide and 0.05% ProClin 300.

Concentration

50 µg/28 µl

Immunogen

synthetic peptide

Background

3–methylcytosine arises by erroneous methylation of DNA through spontaneous exposure to endogenous S–adenosyl methionine (SAM). Since the N3–position in cytosine is protected in double stranded DNA, this phenomenon mainly occurs in single stranded DNA. The resulting 3–mC is toxic and mutagenic and increased levels of 3–mC seem to impair cell proliferation. Therefore, it has to be repaired by the cell. This is established by oxidative demethylation, which is catalyzed by the AlkB protein.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Dot blot analysis using the antibody directed against 3-mC
To demonstrate the specificity of the antibody against 3-mC (cat. No. 25200), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.

Applications

DB (1:400)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human, mouse, broad range

Cross Reactivity

Does not cross–react with 5–methylcytosine, 5–hydroxymethylcytosine, or 5–carboxylcytosine.

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

25200 Lot 150728

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

Anti–3–mC polyclonal antibody

PRODUCTS

SERVICES

RESOURCES

COMPANY