This anti-IL23α (interleukin 23 subunit alpha) is a purified recombinant monoclonal antibody that selectively binds to the p19 subunit of heterodimeric IL-23, which is composed of p19 and IL-12 p40. This antibody has been tested for specific binding affinity to IL23α/IL12β antigen.
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
MW
Heavy Chain: 49 kDa; Light Chain: 24 kDa
UniProt #
Q9NPF7
Background
IL23 (interleukin 23) is a cytokine of the IL12 cytokine family. It is a heterodimeric cytokine with an IL-12p40 subunit and a p19 one. It is produced by macrophages and dendritic cells, and acts as a pro-inflammatory cytokine. It acts on memory T cells, leading to IFNγ (interferon gamma) and IL17 production, and Th17 (T helper type 17) cell proliferation. IL23 is involved in auto-immune and inflammatory disorders, such as psoriasis and arthritis. The use of IL23 targeting antibodies has been ongoing. Ustekinumab, commercialized under the name Stelara™, is one example of the benefits of disrupting the interaction between IL23 and its receptor can be beneficial in the treatment of autoimmune disorders.
Storage/Stability
At least 6 months at -80°C.
Assay Conditions
Functional validation: The antibody was validated by measuring anti-IL23α binding to a IL23α/IL12β antigen (Acro Biosystems #ILB-H52W5) by ELISA. The IL23α/IL12β protein was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 6 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at Room Temperature (RT). After removing the blocking buffer, 50 µl/well of purified anti-IL23α antibody (BPS Bioscience #101453), serially diluted in Blocking Buffer 2, was added for 60 minutes at RT. After 3 more washes, the plate was incubated with Goat Anti-Human IgG Fc (HRP) (Abcam #ab97225), washed, and incubated with Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful for studying the binding of IL23α in ELISA and in cellular assays.
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