Anti-GFRAL Neutralizing Antibody

Catalog #

101351

$395 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Recombinant anti-GFRAL (GDNF Family Receptor alpha Like) neutralizing monoclonal antibody which recognizes GFRAL. This recombinant antibody has been tested for specific binding to purified recombinant human GFRAL (BPS Bioscience #101013) in an ELISA binding assay.

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Synonyms

GDNF family receptor alpha-like, GFRAL, C6orf144, UNQ9356/PRO34128

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

GFRAL:Anti-GFRAL Binding Assay. *Not all lots have been tested for enzyme activity

Anti-GFRAL Neutralization Assay. *Not all lots have been tested for enzyme activity

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

High Chain: 50 kDa; Low Chain: 24 kDa

UniProt #

Q6UXV0

Storage/Stability

At least 12 months at -80°C

Assay Conditions

An ELISA binding assay was performed with this antibody and its target protein, GFRAL (BPS Bioscience #101013). The antibody was coated onto an assay plate overnight at 2 µg/ml at 4°. On the next day, the plate was washed on blocked. Then, serial dilutions of the target protein were added (0 to 200 nM) for 1 hour at room temperature. After washing, HRP-Streptavidin was incubated for 30 minutes at room temperature. Following another wash step, colorimetric HRP substrate was added and quenched with 1N HCL. Then we measured absorbance on a plate reader at 450 nm. EC50 = 2.4 nM

Applications

Useful for studying the binding and neutralization of GFRAL in ELISA and in cellular assays.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Metabolic Enzymes

Regulatory Status

Research Use Only

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Datasheets/Protocols

101351 Lot 220202

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Anti-GFRAL Neutralizing Antibody

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