AMSH Core Domain, His-tag

Catalog #: 81081
Size: 25 µg
Species: Human
Host Species: E. coli
MW: 20.5 kDa
Tag(s): His-tag
a.a: 250–424
AMSHcore (Associated Molecule with the SH3-domain of STAM) is a JAMM domain-containing protein that regulates receptor endosomal sorting of the epidermal growth factor receptor (EGFR). Recombinant AMSH functions as a deubiquitylase in vitro and ablation of AMSH activity by incubation of cells with AMSH siRNA enhances the degradation of EGFR. AMSHcore mediated cleavage of K63 linked ubiquitin chains is enhanced in the presence of its binding partner STAM. AMSHcore contains only the core domain of AMSH protein.
Human AMSH Core Domain recombinant protein, a.a. 250–424, with His-tag, expressed in E. coli.
Associated molecule with the SH3 domain of STAM, STAMBP, MGC126516, MGC126518, STAM-binding protein
Aqueous buffer solution
Storage / Stability:

Store at or below –80°C. Stable as supplied for up to 1 year when stored dessicated at –80°C.

This enzyme cleaves K63-linked ubiquitin chains in vitro. Suggested Substrate: DiUbiquitin K63
1. McCullough, J., et al. AMSH is an endosome-associated ubiquitin isopeptidase. J Cell Biol, 2004. 166(4): p. 487-92.
2. McCullough, J., et al. Activation of the endosome-associated ubiquitin isopeptidase AMSH by STAM, a component of the multivesicular body-sorting machinery. Curr Biol, 2006. 16(2):p. 160-5.
Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Recombinant Protein
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