AAV-DJ SP-B-Luciferase

Catalog #

82135

$550 *

Size: 50 µl x 2

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

AAV-DJ SP-B Luciferase particles transduce firefly (Photinus pyralis) luciferase under the control of a SP-B (Surfactant protein B) promoter that drives luciferase reporter expression mainly in epithelial lung cells.

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Product Data Gallery

Purified AAV-DJ SP-B- Luciferase particles.

Luciferase activity in HEK293 cells transduced with AAV-DJ SP-B-Luciferase particles.

Product Info

Storage and Usage

Citations

Species

AAV-DJ

Supplied As

Two vials (50 µl x 2) of AAV at a titer ≥1 x 10¹² vector genomes/ml. The titer is determined by qPCR and varies with each lot; the exact value will be provided with each shipment.

Purification

The purity of the AAV particles was confirmed to be greater than 90% by staining with One-Step Lumitein™ UV Protein Gel Stain (Biotium, 21005-1L). The purity varies with each lot; the exact value will be provided with each shipment

Formulation

AAV was produced in HEK293-AAV cells and is supplied in PBS-MK (PBS Magnesium-Potassium) buffer containing 0.01% Pluronic F68. Virus particles can be packaged in custom formulations by special request, for an additional fee.

Background

Adeno-Associated Virus-DJ (AAV-DJ) is a synthetic serotype made from eight different wild-type AAV serotypes (AAV2, 4, 5, 8, 9, avian, bovine, and goat AAV) using DNA shuffling. These modifications allow the AAV-DJ serotype to exhibit improved transduction efficiency in vitro and in vivo and infect a broader range of cell types compared to the wild-type serotypes.

Surfactant protein B (SP-B) is a developmentally and hormonally regulated lung protein that is required for normal surfactant function. SP-B is selectively expressed in bronchiolar and alveolar epithelial cells of the lung and it is found to be associated with lipids. Surfactant is a mixture of proteins and lipids that allows proper inflation of the lung and breathing. Deficiency in SP-B results in lethality at birth. The human SP-B promoter fragment directs epitheliallung-cell specific transgene expression.

Storage/Stability

AAV is shipped with dry ice. For long-term storage, it is recommended to store AAV at -80°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle

Applications

Positive control in the transduction of lung epithelial cells.
Optimization of transduction assays and tracking of transgene expression over time.

Shipping Temperature

-80°C

Notes

The AAV-DJ viruses are covered under several patents, including U.S. Patent Nos. 7,588,772, 8,067,014, 8,574,583, and 8,906,387, as well as corresponding foreign patents applications and patent rights. AAV-DJ is used under a license agreement.

Biosafety
Recombinant AAV is inherently replication-deficient and not known to cause any human diseases. Additionally, following transduction, AAV vectors exist episomally and do not integrate into or disrupt the host cell’s genome. AAV requires the use of a Biosafety Level 1 facility. BPS Bioscience recommends following all local, federal, state, and institutional regulations and using all appropriate safety precautions

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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AAV-DJ SP-B-Luciferase

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