Human Fibroblast Growth Factor 10 Recombinant

Catalog #

90133-B

$205 *

Size: 25 µg

Qty

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Description

Recombinant human FGF-10, (Fibroblast Growth Factor 10), is a disulfide-linked monomer protein consisting of 169 amino acids and migrates as an approximately 19 kDa protein under reducing and non-reducing conditions. Source: Optimized DNA sequence encoding human FGF-10 mature chain was expressed in E. coli.

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Synonyms

FGF-10, Keratinocyte Growth Factor 2, KGF2, Fibroblast Growth Factor-10

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥96% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from 0.2 µm filtered PBS solution, pH 7.0.

19 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

40–208

Biological Activity

The ED₅₀ was determined by the dose-dependent proliferation of BaF3 cells expressing FGF receptors, and was found to be <0.5ng/ml., corresponding to a specific activity of 2.0 x 10⁶ Units/mg.

Genbank #

O15520

UniProt #

O15520

Background

FGF-10 is involved in the initial budding as well as the continuous outgrowth of vertebrate limbs, FGF-10 mRNA is expressed preferentially in neurons but not in glial cells and may have a distinct role in the brain. Human FGF-10 is mitogenic for fetal rat keratinizing epidermal cells but not for NIH 3T3 cells. Recombinant FGF-10 induces the proliferation of human urothelial cells in vitro and induces the proliferation of transitional epithelium. FGF-10 is secreted by cultured mouse pre- adipocytes, prevention of FGF-10 signaling inhibits subsequent differentiation. The ability of embryonic fibroblasts derived from FGF-10 knock-out mice to differentiate into adipocytes is also impaired.

References

1. Biol Reprod, Dec 2008, 79: 1226 - 1236.
2. Am. J. Respir. Cell Mol. Biol., Sep 2008, 39: 346 - 355.
3. J. Biol. Chem., Apr 2008, 283: 9308 - 9317.

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Human Fibroblast Growth Factor 10 Recombinant

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