RFP/GFP Safe-Harbor HEK293 Cell Line
RFP/GFP Safe-Harbor HEK293 Cell Line is a stable HEK293 cell line constitutively expressing RFP (Red Fluorescent Protein) and CopGFP (Pontellina plumata green fluorescent protein), which have been stably integrated into the AAVS1 safe harbor locus on chromosome 19.
Expression of RFP is driven by a CMV promoter, whereas CopGFP is driven by an EF1A promoter. The combined DNA fragment (CMV-RFP-bGH Poly A-EF1A-CopGFP-T2A-Puro-SV40 Poly A) (Figure 1) was integrated at the AAVS1 safe harbor locus using CRISPR/Cas9 technology. A monoclonal population was obtained by limiting dilution. This cell line is expected to behave like the parental HEK293 cell line.
Figure 1: Transgene integration at the AAVS1 locus.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
| Name | Ordering Information |
| Thaw Medium 6 | BPS Bioscience #60183 |
The cell line has been screened to confirm the absence of Mycoplasma species.
AAVS1 (also known as the PPP1R12C locus) on human chromosome 19 is a well-validated “safe harbor” site for hosting DNA transgenes. AAVS1 has an open chromatin structure and is transcription competent. Most importantly, disrupting the AAVS1 locus by inserting DNA transgenes has no known adverse effects on the cells. Specifically targeting the AAVS1 locus is a major advantage compared to the random integration obtained using other approaches such as lentivirus infection or cell transfection, which may cause insertional mutagenesis or disrupt important genes or cellular processes. RFP (red fluorescent protein) is a red-orange derivative from Dsred, originally isolated from Discosoma, while GFP (green fluorescent protein) presents green fluorescence, and it was first identified in Aequorea Victoria. Pontellina plumata GFP (CopGFP) was later identified and is a superbright and fast maturation rate. The presence of fluorescent proteins allows for cell identification and quantification by flow cytometry or fluorescence microscopy, and localization studies in vivo, providing easy assay readouts.
Powered by Bioz
San Diego, CA 92121
858-202-1401
Products are for Research Use Only
COMPANY
Copyright © 2024
BPS Bioscience, Inc.
All rights reserved.
Diagrams on this website are created with BioRender.com.