HLA-C*08:02 K562 Cell Line

Catalog #

78974

$6,500 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The HLA-C*08:02 K562 Cell Line is an engineered human lymphoblast K562 cell line expressing human HLA-C*08:02 driven by an EF1a promoter. This cell line was generated by transduction with HLA-C*08:02 Lentivirus (#78930).

This cell line has been validated by flow cytometry and co-culture assays with Jurkat cells expressing KRAS G12D, in the presence of wild type and mutant KRAS peptides

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Cell surface expression of HLA-C*08:02 in HLA-C*08:02 K562 Cell Line by flow cytometry.

Jurkat T cell activation after transduction with KRAS G12D-Specific TCR Lentivirus, using HLA-C*08:02 K562 Cell Line as antigen presenting cells (APCs).

Product Info

Storage and Usage

Citations

Host Cell Line

K562, human lymphoblast cell line, suspension.

Supplied As

Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2E	BPS Bioscience #79638

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Human Leukocyte Antigen-C (HLA-C) is an MHC-I (major histocompatibility complex) heavy chain receptor, composed of HLA-C and β2-microglobulin (B2M). HLA-C is present in all cells and exists as several haplotypes due to the diversity of HLA-C genes. C*08:02 represents one such haplotype. HLA class I present neoantigen-derived peptides to the cell surface, allowing them to be recognized by T cells, via TCR (T cell receptors). Cancer immunotherapy has been taking advantage of that mechanism, by engineering T cells to express TCRs able to recognize specific cancer immunogens. In 2016 the use of HLA-C*08:02-restricted TIL (tumor infiltrating lymphocytes) targeting specifically KRAS (Kirsten rat sarcoma virus) G12D mutation in lung cancer resulted in positive results. A similar approach was pursued in a patient with metastatic pancreatic cancer and resulted in regression of the disease. The study of HLA-C*08:02-restricted TIL expressing TCR against other neoantigens may prove beneficial in cancer therapy. K562 cells are HLA class I and II negative, making them an ideal cellular model to introduce and study specific haplotype responses.

References

Leidner R., et al., 2022 N Engl J Med 386:2112-2119.
Tran E., et al., 2016 N Eng J Med 375:2255-2262.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 2 (BPS Bioscience #60184): RPMI-1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2E (BPS Bioscience #79638): RPMI-1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 0.5 µg/ml of puromycin.

Applications

Useful for studying antigen presentation by HLA-C.
Useful for studying HLA-C restricted T cell responses.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

NK Cells

Regulatory Status

Research Use Only

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HLA-C*08:02 K562 Cell Line

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