HER3 CHO Cell Line

Catalog #

82299

$9,250 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

HER3 CHO Cell Line is a CHO-K1 cell line expressing HER3 (human epidermal growth factor receptor 3) (NM_001982.2, also known as receptor tyrosine-protein kinase erbB-3) under the control of a cytomegalovirus (CMV) promoter. This cell line was generated by lipid-mediated transfection followed by hygromycin selection and limited dilution. Individual clones were screened for HER3 expression levels by flow cytometry, and a clone was selected to generate this cell line.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3, Receptor tyrosine-protein kinase erbB-3, ERBB3

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Product Data Gallery

Flow cytometry analysis of HER3 cell surface expression in HER3 CHO Cell Line.

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent

Supplied As

Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 3	BPS Bioscience #60186
Growth Medium 3B	BPS Bioscience #79529

UniProt #

P21860

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

HER3 (human epidermal growth factor receptor 3) is a transmembrane protein encoded by the ERB3 gene. HER3 is broadly expressed in human tissues however, increased expression of HER3 has been linked to a variety of solid tumors including ovarian, breast, colon, and gastric cancers and correlates with decreased overall survival in colorectal cancer patients. HER3 has the unique property of being inherently catalytically inactive but still being able to participate in ligand binding, forming heterodimers with nearby receptors of the HER family. The formation of these dimers initiates a cascade of downstream signaling steps critical to cell proliferation. The binding and subsequent phosphorylation of HER3 by HER2 or EGFR (epidermal growth factor receptor) has been found to play a role in tumor growth and drug resistance. Due to its cell surface expression in a variety of cancers and correlation with decreased survival, HER3 is a prospective therapeutic target for antibody-drug conjugate (ADC) development.

References

Beji A., et al., 2012 Clin Cancer Res. 18(4):956-68
Criscitiello C., et al., 2021 J Hematol Oncol. 14(1):20
Jaiswal B, et al. 2013 Cancer Cell. 23(5):603-17
Littlefield P, et al., 2014 Sci Signal. 7(354):ra114
Zhou B, et al., 2006 Cancer Cell. 10(1):39-50

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 3 (BPS Bioscience #60186): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 3B (BPS Bioscience #79529): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 500 µg/ml of Hygromycin B.

Applications

Screen therapeutic antibodies and ADCs (antibody-drug conjugates) targeting HER3.
Co-culture assays with HER3-directed CAR-T cells.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cancer Therapy Target

Regulatory Status

Research Use Only

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HER3 CHO Cell Line

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