eGFP Daudi Cell Line

Catalog #

82297

$2,250 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

eGFP Daudi Cell Line is a Daudi cell line engineered to express enhanced GFP (eGFP) driven by an EF1a promoter. This cell line was generated by transduction with Enhanced GFP Lentivirus (Puromycin) (BPS Bioscience #78639).

This cell line has been validated by flow cytometry for eGFP expression.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Flow cytometry analysis of the expression of eGFP in the eGFP Daudi Cell line

Product Info

Storage and Usage

Citations

Host Cell Line

Daudi cells are a Burkitt’s lymphoma B-cell line. Suspension cells.

Supplied As

˃1 x 10⁶ cells in 1 ml of of Cell Freezing Medium (BPS Bioscience, #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2K	BPS Bioscience #78078

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Daudi is a B lymphoplast cell line isolated from a human Burkitt lymphoma patient, which is commonly used in the study of leukemia. Burkitt lymphoma is a type of B-cell non-Hodgkin lymphoma, which is a rare and fast-growing cancer with origins often in the abdomen or spleen. This cell line is used as target cells in studies involving T cells. The presence of eGFP (enhanced green fluorescent protein) allows for easy assay readouts.

Storage/Stability

Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. To formulate a comparable but not BPS validated media, formulation components can be found below.

Media Required for Cell Culture
Thaw Medium 2 (BPS Bioscience #60184): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2K (BPS Bioscience #78078): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin, plus 0.25 μg/ml of Puromycin.

Applications

In vitro and in vivo fluorescence imaging.
Use as target cells in CAR-T co-culture killing assays.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cancer Therapy Target

Regulatory Status

Research Use Only

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eGFP Daudi Cell Line

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