AAV6 Luciferase-mCherry
Catalog #
78475
$545
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Description
Adeno-Associated Virus serotype 6 (AAV6) appears to be related to AAV1 by sequence analysis and shows the best transduction efficiency in pancreatic beta-cells compared to other AAV serotypes.
These AAV6 particles constitutively express the firefly (Photinus pyralis) luciferase and mCherry genes connected via a T2A linker, under the control of a CMV promoter. The T2A self-cleaving peptide (derived from Thosea asigna virus 2A) leads to the efficient cleavage of the transcript and expression of luciferase and mCherry as two separate proteins.
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Product Info
Storage and Usage
Citations
Species
Wild-type AAV Serotype 6
Supplied As
Two vials (50 µl x 2) of AAV at a titer ≥1 x 1011 vector genomes/ml. The titer is determined by qPCR and will vary with each lot; the exact value will be provided with each shipment.
Purification
The purity of the AAV particles was confirmed to be greater than 90% by staining with One-Step Lumitein™ UV Protein Gel Stain (Biotium #21005-1L). Purity will vary with each lot; the exact value will be provided with each shipment.
Formulation
AAV6 was produced in HEK293-AAV cells and is supplied in PBS-MK (PBS Magnesium-Potassium) buffer containing 0.01% Pluronic F68.