CD36 Knockout THP-1 Cell Line

Catalog #

82190

$6,500 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

CD36 Knockout THP-1 Cell Line is a THP-1 cell line where CD36 (Cluster of Differentiation 36) has been genetically removed from THP-1 cells using CRISPR/Cas9 genome editing via the use of a lentivirus encoding CRISPR/Cas9 gene and sgRNA (single guide RNA) targeting human CD36.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Genomic sequencing of CD36 in the CD36 Knockout THP-1 Cell Line.

CD36 expression in CD36 Knockout THP-1 cells

Product Info

Storage and Usage

Citations

Host Cell Line

THP-1, human monocyte, suspension

Supplied As

Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2M	BPS Bioscience #78181

UniProt #

P16671

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

CD36 is a membrane glycoprotein present on platelets, mononuclear phagocytes, adipocytes, hepatocytes, myocytes, and some epithelia. On microvascular endothelial cells, CD36 is a receptor for thrombospondin-1 and related proteins and functions as a negative regulator of angiogenesis. On phagocytes, through its functions as a scavenger receptor recognizing specific oxidized phospholipids and lipoproteins, CD36 participates in internalization of apoptotic cells, certain bacterial and fungal pathogens, and modified low-density lipoproteins, thus contributing to inflammatory responses and atherothrombotic diseases. CD36 also binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption and possibly contributing to the pathogenesis of metabolic disorders, such as diabetes and obesity. CD36 in many cells is localized in specialized cholesterol-rich membrane microdomains and may also interact with other membrane receptors, such as tetraspanins and integrins. Identification of the precise CD36 signaling pathways in specific cells elicited in response to specific ligands may yield novel targets for drug development.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 2 (BPS Bioscience #60184): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2M (BPS Bioscience #78181): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 µg/ml of Puromycin.

Applications

Study cellular mechanisms and diseases related to CD36 knockout.
Introduce further CRISPR/Cas9-based genetic manipulations in order to understand the interplay between CD36 and other partners and pathways.

Shipping Temperature

-80°C

Notes

The CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights.

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immune Cell Signaling

Regulatory Status

Research Use Only

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CD36 Knockout THP-1 Cell Line

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