ATAD2A Inhibitor Screening Assay Kit
Catalog #
32601
$1,025
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Description
The ATAD2A Inhibitor Screening Assay Kit is designed to measure the inhibition of ATAD2A binding to its substrate. The key to the ATAD2A Inhibitor Screening Assay Kit is a specific binding of the ATAD2A bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing ATAD2A and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
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Synonyms
KIAA1240, AAA domain containing 2B (variant 1), activity assay kit
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Product Data Gallery
Product Info
Storage and Usage
Citations
Assay Kit Format
AlphaLISA®
Supplied As
Kit comes in a convenient AlphaLISA® format, with biotinylated histone peptide substrate, assay buffer, detection buffer and purified GST-tagged ATAD2A bromodomain to perform a total of 384 enzyme reactions.
Materials Required But Not Supplied
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Glutathione AlphaLISA® Acceptor Beads, 5 mg/ml (PerkinElmer #AL109C)
AlphaScreen® Streptavidin-conjugated donor beads, 5 mg/ml (PerkinElmer #6760002)
Optiplate-384 (PerkinElmer #6007290)
AlphaScreen® microplate reader
Adjustable micropipettor and sterile tips
Glutathione AlphaLISA® Acceptor Beads, 5 mg/ml (PerkinElmer #AL109C)
AlphaScreen® Streptavidin-conjugated donor beads, 5 mg/ml (PerkinElmer #6760002)
Optiplate-384 (PerkinElmer #6007290)
AlphaScreen® microplate reader
Adjustable micropipettor and sterile tips
Format
COMPONENTS:
UniProt #
Q6PL18
References
Filippakopoulos, P., et al., Cell 2012; 149:214.