CBL-B, GST-Tag (Human)

Catalog #: 80415
Size: 100 µg
Species: Human
Host Species: E. coli
MW: 72 kDa
Genbank #: NM_170662
Tag(s): N-terminal GST-Tag
a.a: 39 – 426
Human Casitas B-lineage lymphoma proto-oncogene b (CBL-B), also known as E3 ubiquitin-protein ligase CBL-B, and RING finger protein 56, GenBank Accession No. NM_170662, a.a. 39 – 426 with N-terminal GST-Tag expressed in an E. coli expression system. MW=72 kDa.
E3 ubiquitin-protein ligase CBL-B, RING finger protein 56, CBL, CBLB
Assay Conditions:
10 uL of reaction mixture contains 50 mM Tris, pH 7.4, 5 mM MgCl2, 0.01% Tween, 1 mM DTT, 0.3 µM Ub[Biotin], 100 nM Ube1, 1 mM ATP, 2 µM of UBCH5b, and various amount of CBL-B. The reaction was initiated with the addition of ATP, and incubated at 30° C for 3 hours. Tb labeled anti-GST antibody donor and XL665 labeled acceptor was added and incubated at room temperature for an hour.
40 mM HEPES pH 7.4, 120 mM NaCl, 20% glycerol.
Storage / Stability:
At least 6 months at –80˚C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
1. Jeon, M.S., et al., Immunity. 2004; 21(2): 167-177.

2. Chiang, Y.J. et al., Nature. 2000; 403: 216-220.

Avoid freeze/thaw cycles
Scientific Category:
Ubiquitin E3 ligase
Product Type:
Recombinant Protein
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