TNK1, GST-Th-Tag Recombinant

Catalog #

80019

$310 *

Size: 100 μg

Qty

Bulk/Custom

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Description

TNK1 (Tyrosine Kinase Non Receptor 1), also known as CD38 Negative Kinase 1 (GenBank Accession No. NM_003985), a.a. 106-390, with N-terminal GST-tag and Thrombin Cleavage Site, MW = 57 kDa, expressed in Sf9 cells via a baculovirus expression system.

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Synonyms

CD38 Negative Kinase 1, Tyrosine Kinase Non Receptor 1, Mitogen-Activated Protein Kinase 1, MAPK1, CD36 Molecule, CD36, RAF1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 9mM Glutathione

57 kDa

Amino Acids

106-390

Specific Activity

≥3.5 pmol/min/ug

Genbank #

NM_003985

UniProt #

Q13470

Tag(s)

GST-Th-Tag

References

1. Felschow, D. (2000). BBRC. 273(1):294-301.

2. Azoitei, N. et al. (2010). Oncogene. 26: 6536-6545.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was done in Kinase buffer containing 1 mM DTT using CSKtide substrate (0.2 mg/ml) and 10 μM ATP. Reaction was done at 30°C for 50 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega)

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C (dry ice)

Warnings

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Scientific Category

Kinase

Regulatory Status

Research Use Only