Sirtuin 5, GST-tag Recombinant

Catalog #

50016

$440 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human Sirtuin 5 (GenBank Accession No. NM_012241), full length with N-terminal GST-tag, MW= 59.8 kDa, expressed in an E. coli expression system.

This product has been cited 6 times.

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Synonyms

Sirtuin5, SIRT5

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Sirtuin5 Activity

SIRT5 (Sirtuin5) inhibition curve using a fluorogenic assay (#50085). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations6

Species

Human

Host Species/Expression System

E. coli

Purity

≥50%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione and 10% glycerol.

59.8 kDa

Amino Acids

full length

Specific Activity

≥2.0 pmole/min/µg

Genbank #

NM_012241

UniProt #

Q9NXA8

Tag(s)

N-terminal GST-tag

References

1. Mahlknecht, U. et al., (2006) Cytogenet. Genome Res. 112, 208-212.
2. Frye, R.A. (2000) Biochem. Biophys. Res. Commun. 273, 793-798.

Storage/Stability

>6 months at -80°C.

Assay Conditions

25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, 0.1 mg/ml BSA, 200 µM SIRT5 substrate 5 (Catalog #50401), 500 µM NAD+ and Sirtuin 5 (15.6 - 1000 ng). Incubation condition: 30 min at 37°C followed by treatment with 50 µl of SIRT developer (Catalog # 50089) for 15 min at RT. Fluorescence intensity is measured at ex360/em460.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Deacetylase

Regulatory Status

Research Use Only

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Datasheets/Protocols

50016_Lot130110.pdf

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