SIK (SNF1LK), His-tag Recombinant

Catalog #
40274
$410 *
Size: 10 µg
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Description

Recombinant human SIK (GenBank Accession No. NM_173354), a.a. 1-303, with N-terminal His-tag, MW = 36 kDa, expressed by Sf9 insect cells via a baculovirus expression system.

Synonyms
SNF1LK, MSK, SIK1, salt-inducible protein kinase 1, SNF1-like kinase
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
Sf9 insect cells
Purity

≥80%

Format
Aqueous buffer solution
Formulation
50 mM sodium phosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol
MW
36 kDa
Amino Acids
1–303
Specific Activity
290 nmol/min/mg
Genbank #
NM_173354
UniProt #
P57059
Tag(s)
N-terminal His-tag
Background
SIK is a protein kinase that is involved in regulating AMPK-related kinases (1). SIK may mediate the physiological effects of LKB1, including its tumour suppressor function. SIK is also involved in signaling by various proteins like STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4 and MELK that are related to AMPK. Activation of SIK1 by phosphorylation on thr322 can lead to an increase in the catalytic activity of sodium/potassium ATPase alpha subunit at the plasma membrane (2). This results in an increase in intracellular sodium in intact mammalian cells.
References
1.Takemori, H. Et al: TORC-SIK cascade regulates CREB activity through the basic leucine zipper domain. FEBS J. 2007; 274 (13): 3202–9.
2.Sjostrom, M.; SIK1 is part of a cell sodium-sensing network that regulates active sodium transport through a calcium-dependent process. Proc. Nat. Acad. Sci. 104: 16922-16927, 2007.