SENP2, His-tag Recombinant

Catalog #

81095

$1,150 *

Size: 1000 units

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Description

Active recombinant SENP2 (SUMO Protease 2). This construct contains an N-terminal His-tag. This protein was affinity purified.

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Synonyms

SUMO Protease 2

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Product Data Gallery

SDS-PAGE Coomassie Staining

RP-HPLC

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90% by SDS-PAGE and RP-HPLC

Format

Aqueous buffer solution

Formulation

50 mM HEPES, pH 7.5, 150 mM NaCl, 10% glycerol

28 kDa

Specific Activity

> 1 x 10⁵ U/mg, 1 U will cleave >90 µg of hSUMO3-GFP in 1 hr at 37°C

UniProt #

Q9HC62

Tag(s)

N-terminal His-tag

Background

SUMO Protease 2, a highly active and robust recombinant protease, cleaves hSUMO3 from recombinant fusion proteins. Unlike thrombin, EK, or TEV protease, which recognize short, linear sequences, SUMO Protease 2 recognizes the tertiary structure of huSUMO3. As a result, SUMO Protease 2 will not cleave within the fused protein of interest.

References

1. MarblestoneJG, et al. (2006). "Comparison of SUMO fusion technology with traditional gene fusion systems: Enhanced expression and solubility with SUMO," Protein Science, 15:182-9.
2. Butt TR, Edavettal SC, Hall JP, Mattern MR (2005). "SUMO fusion technology for difficult-to-express proteins," Protein Expr Purif., 43(1):1-9.
3. Zuo X, et al. (2005). "Enhanced expression and purification of membrane proteins by SUMO fusion in Escherichia coli.," J. Struct. Funct. Gen., 6:103-11.
4. Malakhov MP, et al. (2004). "SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins," J. Struct. Funct.Gen., 5:75-86.

Storage/Stability

Store at or below –80°C. Stable as supplied for up to 1 year when stored dessicated at –80°C.

Assay Conditions

SUMO3-containing protein should be purified and dialyzed with PBS, pH 7.4 or 20 mM Tris Buffer and 150 mM NaCl pH 8. Incubation with Senp2 is then performed by adding 1 unit of Senp2 per each 10-100 µg of SUMO-3-containing protein and adding DTT to reach a 2 mM final concentration. Reactions can be incubated at 30°C for 1 hour with gentle agitation (no vortexing) or overnight at 4°C.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for removal of hSUMO3 from recombinant proteins over a range of temperature (30°C optimal) and ionic strength, and from pH 5.5 to 9.5.

Shipping Temperature

-80°C (dry ice)

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Ubiquitination (SUMOylation)

Regulatory Status

Research Use Only

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Datasheets/Protocols

81095 Lot 231113

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