PDE4A10, GST-tag

Catalog #: 60038
Size: 10 µg
Species: Human
Host Species: Sf9 insect cells
MW: 117 kDa
Genbank #: NM_001111309
Tag(s): N-terminal GST-tag
a.a: 2–825(end)
Human PDE4A10, also known as PDE4A protein isoform 6, transcript variant 3 (GenBank Accession No. NM_001111309), a.a. 2-825(end) with N-terminal GST-tag, MW= 117 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
PDE4A protein isoform 6, transcript variant 3, PDE4A10
Specific Activity:
≥ 4,400 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay Conditions:
Assayed using BPS's PDE4A Assay Kit, Cat. # 60340. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4A10 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol and 3 mM DTT.
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
1. McCahill, A., et al. Cell Signal. 2008. Nov; 20(11):2071-83.
2. McPhee, I., et al., Cell Signal. 2001. Dec; 13(12):911-8.
Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Recombinant Protein
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