PDE2A V1 (Mouse), GST-tag

Catalog #: 60017
Size: 5 μg
Species: Mouse
Host Species: Sf9 Insect Cells
MW: 130 kDa
Genbank #: NM_001143848
Tag(s): N-terminal GST-tag
a.a: 2-939 (end)
Murine PDE2A, variant 1 (GenBank Accession No. NM_001143848), a.a 2-939 (end), with N-terminal GST tag, MW=132 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Specific Activity:
≥20,000 pmole/min/µg. One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C. Assay condition: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of mouse PDE2A, mouse PDE4C and mouse PDE1B at 37ºC for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20 and 20% glycerol.
Storage / Stability:
At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
1. Sadhu, K., et al., JHC 47(7), 889-894 (1999).

2. Iffland, A., et al., Biochemistry 44 (23), 8312-8325 (2005).

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