p38γ (p38gamma), GST-tag Recombinant

Catalog #

40144

$410 *

Size: 10 µg

Qty

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Description

Recombinant human P38γ also known as MAPK12 (mitogen-activated protein kinase 12), encompassing full length amino acids. This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified and is kinase active.

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Synonyms

ERK3; ERK6; SAPK3; PRKM12; MAPK12, p38

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

p38γ (GST-Full Length)

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol

71 kDa

Amino Acids

full length

Genbank #

NM_002969

UniProt #

P53778

Tag(s)

N-terminal GST-tag

Background

p38γ is a member of the p38 MAPK family which is activated in response to stress (1). p38γ gene was mapped to 22q13.3 and functions as a signal transducer during differentiation of myoblasts to myotubes. Enforced localization of p38γ in the nucleus or cytoplasm markedly attenuates the ability of the kinase to induce cell cycle arrest in fibroblasts. p38γ increases basal glucose uptake and decreases DNP- and contraction-stimulated glucose uptake, partially by affecting levels of glucose transporter expression in skeletal muscle (2).

References

1. Li, Z. et al: The primary structure of p38-gamma: a new member of p38 group of MAP kinases. Biochem. Biophys. Res. Commun. 228: 334-340, 1996.
2. Ho, RC. et al: p38gamma MAPK regulation of glucose transporter expression and glucose uptake in L6 myotubes and mouse skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2004 Feb; 286(2):R342-9.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

p38γ kinase activity was measured P38 Sub synthetic peptide (IPTTPITTTYFFFKKK) diluted in water to a final concentration of 1 mg/ml. Increasing amounts of kinase were mixed with a final concentration of 200 µg/ml peptide substrate in a buffer consisting of 5 mM MOPS pH 7.2, 2.5 mM β-glycerol-phosphate, 1 mM EGTA, 5 mM MgCl₂, 1 mM EGTA and 0.4 mM EDTA with 50 ng/µl BSA (bovine serum albumin) and .25 mM fresh DTT (final concentrations). The reaction was initiated by adding 5 µl [33P]-ATP (1 µCi/sample) mixture containing 0.25 mM non-radioactive ATP The blank was determined from a “no substrate” sample.

The reaction was incubated for 15 minutes at 30°C and terminated by spotting 20 μl of the mixture onto phosphocellulose paper strips that were fixed in 1% phosphoric acid and washed three times. Radioactivity was determined using a scintillation counter.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40144 Lot 221018

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p38γ (p38gamma), GST-tag Recombinant

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