NSP10/NSP16 Complex (SARS-CoV-2) Recombinant

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Catalog #
100747
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Description

Complex of recombinant SARS-CoV-2 proteins NSP10 and NSP16. These recombinant proteins are co-expressed and affinity purified. Recombinant SARS-CoV-2 NSP10 (Nonstructural protein 10), full-length encompassing amino acids 1-139(end). This construct contains an N-terminal FLAG-tag. Recombinant SARS-CoV-2 NSP16 (Nonstructural protein 16), full-length encompassing amino acids 1-298(end). This construct contains an N-terminal His-tag, (6xHis).

Synonyms
nonstructural protein 10, nonstructural protein 16
Product Info
Storage and Usage
Citations
Species
SARS-CoV-2
Construct
NSP10 (FLAG-1-139(end)) / NSP16 (His-1-298(end)) (SARS-CoV-2)
Host Species/Expression System
HEK293
Purity

≥90%

Format

Aqueous buffer solution

Formulation

20 mM HEPES, pH 7.4, 150 mM NaCl, 4 mM MgCl2, variable μg/ml FLAG, and 10% glycerol

MW
NSP10: 16 kDa; NSP16: 34 kDa
Amino Acids
NSP10: 1-139(end); NSP16: 1-298(end)
Genbank #
NSP10: YP_009742617; NSP16: YP_009725311
Tag(s)
NSP10: N-terminal FLAG-tag; NSP16: N-terminal His-tag
References

1. Rosas-Lemus, M., et. al., Europe PMC. DOI: 10.1101/2020.04.17.047498, 2020.
2. Krafcikova, P. et. al, BioRxiv. DOI: 10.1101/2020.05.15.097980, 2020.