NSD1 Chemiluminescent Assay Kit

Catalog #

53010

$920 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The NSD1 Direct Activity Chemiluminescent Assay Kit is designed to measure NSD1 activity for screening and profiling applications. The key to the NSD1 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S- adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against NSD1? Check out our Methyltransferase Screening Services.

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Synonyms

Nuclear Receptor Binding SET Domain Protein 1, KMT3B, NSD1, NSD

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

Kit comes in a convenient format, with 96-well plate precoated with histone H3 substrate, reagents, and purified NSD1 enzyme for 96 enzyme reactions.

Materials Required But Not Supplied

TBST buffer (1 x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format

Catalog Number	Component	Amount	Storage
51024	NSD1 human enzyme	100 µg	-80°C	Avoid freeze/ thaw cycles!
52120	400 μM S-adenosylmethionine	500 µl	-80°C
52140P2	Primary antibody 16-2	12.5 µl	-80°C
52131H	Secondary HRP-labeled antibody 2	10 µl	-80°C
52193Z	4x HMT assay buffer 7	3 ml	-20°C
52100	Blocking buffer 4	50 ml	+4°C
	HRP chemiluminescent substrate A (translucent bottle)	6 ml	+4°C
	HRP chemiluminescent substrate B (brown bottle)	6 ml	+4°C
	96-well plate precoated with histone substrate	1	+4°C

UniProt #

Q96L73

References

Dillon S.C., et al. 2005. Genome Biology 6:227.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

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NSD1 Chemiluminescent Assay Kit

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