TYK2 (Tyrosine Kinase 2) Assay Kit

Catalog #
79527
$560 *
Size: 96 reactions
Qty
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Description

Janus kinase (JAK) is a family of intracellular, nonreceptor tyrosine kinases that play an important role in cytokine-mediated signaling. This family includes four protein kinases; Jak1, Jak2, Jak3 and Tyk2. Various studies have suggested that regulation of Jak activity should be a promising approach to combat numerous diseases such as immune deficiencies and cancers. The TYK2 (Tyrosine Kinase 2) Assay Kit is designed to measure TYK2 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The TYK2 (Tyrosine Kinase 2) Assay Kit comes in a convenient 96-well format, with enough purified recombinant TYK2 enzyme, TYK2 substrate peptide (IRS-1tide), ATP and kinase assay buffer for 100 enzyme reactions.

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Synonyms
IMD35, JTK1, tyrosine kinase 2, Non-receptor tyrosine-protein kinase biochemical assay kit, JAK family
Product Info
Storage and Usage
Citations
Assay Kit Format
Luminescent
Supplied As
The TYK2 (Tyrosine Kinase 2) Assay Kit comes in a convenient 96-well format, with enough purified recombinant TYK2 enzyme, TYK2 substrate peptide (IRS-1tide), ATP and kinase assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format
Catalog
Number

Components

Amount 

Storage
40285 TYK2 1 µg -80°C

Avoid multiple
freeze/thaw
cycles!
79334 5x Kinase assay buffer 1 1.5 ml -20°C
79686 ATP (500 μM) 100 µl -20°C
79519 10x IRS1-tide 500 µl -20°C
79696 96-well plate, white 1 Room Temp.  
UniProt #
P29597
References

Alicea, N.L. et al., Current Drug Targets 12(4):546-555 (2011)