RET Assay Kit

Catalog #
79566
$535 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

RET is a receptor kinase for GDNF (glial cell-line derived neurotrophic factor) family ligands (GFLs). Activation of wild-type RET is important in several cancers where it contributes to tumor progression through various mechanisms. In addition, it has been shown that activating mutation as well as RET rearrangement that leads constitutively active protein plays a significant role in various cancer types. Importantly, small molecule inhibitors of RET have been clinically proved as a promising therapeutic reagent in medullary thyroid cancer and are being evaluated for other types of cancers related to RET overexpression or mutation. The RET Assay Kit is designed to measure RET activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The RET Assay Kit comes in a convenient 96-well format, with enough purified recombinant RET enzyme, RET substrate peptide (IRF-1Rtide), ATP and kinase assay buffer for 100 enzyme reactions.

Need us to run inhibitor screens or profile your compounds against RET? Check out our Kinase Screening Services.

Product Info
Storage and Usage
Citations
Assay Kit Format
Luminescent
Supplied As
The RET Assay Kit comes in a convenient 96-well format, with enough purified recombinant RET enzyme, RET substrate peptide (IRF-1Rtide), ATP and kinase assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format
Catalog
Number

Reagent

Amount 

Storage
40267 RET 15 µg -80°C
Avoid 

multiple
freeze/thaw
cycles!
79334 5x Kinase assay buffer 1 1.5 ml -20°C
79686 ATP (500 μM) 100 µl -20°C
79567 IGF-1Rtide (1 mg/ml) 500 µl -20°C
79696 96-well plate, white 1 Room Temp.  
UniProt #
P07949
References
Mulligan, L. M., Nature Reviews Cancer 14:173-186 (2014)